mouse anti n cadherin  (Developmental Studies Hybridoma Bank)

Journal: International Dental Journal

Article Title: Frog-Derived Peptide RL-RF10 Facilitates Gingival Repair and Regeneration Through the Integrin αvβ3/p38/Snail1 Axis

doi: 10.1016/j.identj.2025.109337

Figure Lengend Snippet: RL-RF10 promotes p-EMT through integrins αv and β3. (A) Western blot analysis of p-EMT marker proteins in HOKs treated with RL-RF10. (B) Quantitative analysis of protein expression. (C) Immunofluorescence staining for vimentin and N-cadherin proteins, with fluorescence intensity represented in 3D surface plots. Scale bar = 20 μm. (D) Western blot analysis of integrins αv and β3. (E) Quantitative analysis of protein expression. (F) Immunofluorescence staining for integrins αv and β3, with 3D surface plots showing fluorescence intensity. Scale bar = 20 μm. Data are presented as mean ± SEM ( n = 3, * P < .05, ** P < .01).

Article Snippet: The sliced tissues were incubated with the anti-N-cadherin mouse antibody (Servicebio).

Techniques: Western Blot, Marker, Expressing, Immunofluorescence, Staining, Fluorescence

Journal: International Dental Journal

Article Title: Frog-Derived Peptide RL-RF10 Facilitates Gingival Repair and Regeneration Through the Integrin αvβ3/p38/Snail1 Axis

doi: 10.1016/j.identj.2025.109337

Figure Lengend Snippet: RL-RF10 enhances gingival soft tissue regeneration in vivo. (A) Establishment of a New Zealand White Rabbit model with gingival tissue defects (created using BioRender). (B) Wound healing rates of gingival defects treated with RL-RF10. (C) H&E staining of tissue sections at different healing stages. Overview images (scale bar = 500 μm) with corresponding magnified views (scale bar = 200 μm) aligned in a right-hand column, linked by dashed lines. (D) Masson’s trichrome staining showing collagen fiber deposition during wound healing. For each group, the upper row displays overview images at sequential time points (scale bar = 500 μm), and the lower row directly beneath shows higher-magnification views of the corresponding boxed regions above, connected by dashed lines (scale bar = 200 μm). (E) Immunohistochemistry (IHC) analysis of N-cadherin expression in tissue sections. n = 3, * P < .05, ** P < .01.

Article Snippet: The sliced tissues were incubated with the anti-N-cadherin mouse antibody (Servicebio).

Techniques: In Vivo, Staining, Immunohistochemistry, Expressing